Protein Modification Could Push Cellulosic Biofuel Forward

Protein Modification Could Push Cellulosic Biofuel Forward

Biochemistry researchers modify protein complex resulting in better yielding crops for cellulosic biofuel production

Production of cost-efficient cellulosic biofuels has been limited by lignin, which binds tightly to the cellulose found in plants' cell walls. But a Purdue researcher says a modifying a certain protein complex in the plant could lead to a plant that lends itself to cellulosic biofuel production without limiting yield.

Biochemistry professor Clint Chapple and fellow researchers generated a mutant Arabidopsis plant that can be converted to fermentable sugars. But it doesn't display growth stunting like similar mutants.

Biochemistry researchers modify protein complex resulting in better yielding crops for cellulosic biofuel production (Purdue photo)

"This study opens the door to a whole new set of technologies we never could have imagined," Chapple said. "This finding is not the silver bullet that will suddenly make the wide-scale production of cellulosic biofuels possible, but it is a very important step forward."

How it works
Making cellulosic biofuels from the sugars in the plants' cell walls requires freeing cellulose from lignin so that it can be broken down fermented into fuel. That requires expensive and complicated pretreatment processes.

To avoid the pretreatment, scientists have probed ways of genetically modifying plants to weaken lignin's grip, but that often leaves them dwarfed and low-yielding.

"We've always known we couldn't eliminate lignin entirely," Chapple said. "If there isn't enough lignin in the cell walls, the plant's water conducting system will collapse. For the plants, it's like trying to drink a milkshake through a paper straw. They need the strength lignin provides to pull water up from their roots."

Related: Cellulosic Biofuel Production Continues, But Remains Under Target

The challenge, Chapple said, was to find a way of preserving lignin's key structural functions while preventing it from interfering with the use of cellulosic materials.

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Chapple and his fellow researchers took an Arabidopsis mutant plant in which the lignin biosynthetic pathway had been interrupted – and was, therefore, weak and dwarfed – and made two additional mutations by knocking out two plant genes known as MED5a and MED5b.

The triple mutation resulted in a healthy plant with normal growth and wild-type levels of lignin.

"When I saw this plant, I thought it couldn't possibly be true," Chapple said. "We thought plants like this would have too many architectural problems to grow properly. But what this indicates is that plants with a blocked lignin pathway can grow, they're just choosing not to."

The study showed that suppressing the MED5 genes took away the mutant plant's ability to sense the disruption in its lignin biosynthetic pathway. It responded by growing normally and producing a highly unusual type of lignin that interfered far less with the breakdown of cell walls into their sugar components than lignin in wild-type plants.

Related: Cellulosic Ethanol A 'Good Environmental Fit' For Corn Growers

When treated with enzymes, the triple mutant yielded significantly more glucose than normal plants.

"We've never worked with plant material like this before," Chapple said. "It gives up its sugars quite easily and eliminates the need for pretreatment, which is a big component of the expense of making cellulosic biofuels."

Broader applications?
While lignin is considered a waste material in current biofuels production, the simple composition of the novel lignin – which is made up almost exclusively of a single type of alcohol – could make it a potential fuel source in the future, Chapple said.

The discovery could also lead to the development of more digestible forage crops, which could improve weight gain in livestock.

Chapple cautioned that suppressing genes in MED5 is not necessarily a quick fix to the complications of producing cellulosic biofuels. Mutations in MED5 can negatively affect other important plant processes. But the study gives important insights into plant metabolism and how plant cell walls are made, he said.

"Learning that this pathway exists gives us an opportunity to find out how it works. These plants aren't dwarfed for the reason we thought they were. Now we can reexamine other ways of modifying lignin that we'd thought were useless."

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